In vitro prevention by ACE inhibitors of cataract induced by glucose
نویسندگان
چکیده
Department of Pharmacology, Objectives: To study, the anticataract activity of lisinopril and enalapril on cataract inGrant Medical College, duced by glucose, in goat lenses. Mumbai, Materials and Methods: Goat lenses were incubated in artificial aqueous humor contain *Ranbaxy Laboratories Ltd., **Department of Biochemistry, ing 55 mM glucose (cataractogenesis) with lisinopril or enalapril in different concentrations B.J. Medical College, Pune, at room temperature for 72 h. Biochemical parameters studied in the lens were electrolytes ***Department of (Na +, K+), Na+-K+-ATPase activity, malondialdehyde (MDA) and proteins. Pharmacology, B.J. Medical Results: Glucose induced opacification of goat lens began 8-10 hrs after incubation and College, Pune. was complete in 72-80 hrs. Cataractous lenses showed higher Na+, MDA (P<0.001), lower Na+-K+-ATPase activity, and water-soluble protein content. Lenses treated with lisinopril or Received: 16.5.2005 enalapril in concentrations of 1, 5, and 10 ng/ml showed higher protein (total and water Revised: 13.6.2005 soluble proteins) content and prevented formation and progress of cataract by glucose, as Accepted: 21.10.2005 evidenced by biochemical parameters. Correspondence to: Conclusion: The anticataract activity of lisinopril and enalapril may be because of the Deepak G. Langade antioxidant and free radical scavenging activity, as evidenced by a decrease in MDA in E-mail: [email protected] treated lenses. Further in-vitro and in-vivo studies in various experimental models and long term clinical trials are required to validate the anticataract activity of ACE-inhibitors.
منابع مشابه
Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy
Objective(s):The neuroprotective effect offruit aqueous extract of Ziziphus jujuba Lam on glucose-induced neurotoxicity in PC12 cells as an appropriate in vitro model of diabetic neuropathy was investigated. Materials and Methods: Cell viability was determined by the MTT assay. Cellular reactive oxygen species (ROS) generation was measured by DCFH-DA analysis. Cleaved caspase-3, a biochemical p...
متن کاملPrevention of selenite-induced cataractogenesis by hydroalchoholic extract of Echium amoenum: An experimental evaluation of the Iranian traditional eye medication
Echium amoenum is an Iranian indigenous medicinal plant. In this research the effect of hydroalchoholic extract of this plant on sodium-selenite induced cataract formation was evaluated. Fifty five white rat pups were selected and divided into six groups. In first group (control 1) no elements injected. Second group (control 2) was received normal saline on 11-18 postpartum. Hydroalchoholic ext...
متن کاملMelatonin Reduces Cataract Formation and Aldose Reductase Activity in Lenses of Streptozotocin-induced Diabetic Rat
Background: The relationship between the high activity of aldose reductase (AR) and diabetic cataract formation has been previously investigated. The purpose of the present study was to determine the preventing effect of melatonin on streptozotocin (STZ)-induced diabetic cataract in rats.Methods: 34 adult healthy male Sprague-Dawely rats were divided into four groups. Diabetic control and diabe...
متن کاملAscorbic Acid Diminishes Developmental Damages Induced by High Glucose in Mouse Preimplantation Emberyos in vitro
متن کامل
Inhibition of Angiotensin-Converting Enzyme Reduces Cerebral Infarction Size in Experimental-Induced Focal Cerebral Ischemia in the Rat
Background: The role of Renin Angiotensin System (RAS) in ischemic/reperfusion (I/R) injuries is not fully elucidated. Furthermore, it is not clear whether inhibition of RAS by Angiotensin-Converting Enzyme (ACE) inhibitors has beneficial effects in terms of protecting the brain from I/R injuries. In this study enalapril is used as an ACE inhibitor to evaluate the role of RAS in I/R injuries in...
متن کامل